En tres de los 21 casos que estudiaron Joshi Alumkal y su equipo, la expresión génica de los tumores de próstata cambió después de recibir enzalutamida y ya no tenían el receptor de andrógenos (que se muestra en rojo) como motor. Crédito: Cortesía de Joshi Alumkal
Las biopsias de los mismos pacientes antes y después del tratamiento revelan cómo un fármaco específico reprograma los tumores de cáncer de próstata.
Durante más de una década, se han utilizado para tratar el cáncer de próstata avanzado medicamentos como la enzalutamida (también conocida por el nombre comercial Xtandi) que impiden que las hormonas masculinas activen el receptor de andrógenos. Aunque en la mayoría de los casos son exitosos, a veces estos medicamentos finalmente dejan de funcionar. Sin embargo, existe una comprensión limitada de cómo se produce este cambio.
Un nuevo estudio sugiere que los inhibidores de los receptores de andrógenos pueden reconfigurar y remodelar fundamentalmente el funcionamiento de los tumores de próstata. De hecho, en algunos casos, incluso pueden hacer que los tumores sean más agresivos. Estos hallazgos del Centro de Cáncer Rogel de la Universidad de Michigan se publicarán hoy (15 de septiembre de 2022) en la revista
Alumkal was interested in learning what was present in these tumors to begin with and understanding what happened after tumors started to grow on enzalutamide treatment.
He and colleagues recruited patients for a longitudinal study to obtain metastatic biopsies before enzalutamide treatment and at the time the tumor became resistant to treatment. Serial biopsies from 21 patients were collected by his team, enabling them to understand the workarounds in the tumor from each patient.
According to Alumkal, this is the largest collection of matched metastatic biopsies before and after enzalutamide. “To understand resistance to drugs, researchers often collect samples from some patients before treatment and from a different group of patients whose tumors are treatment resistant. However, that approach is much less precise because there could be other significant differences between those patients. You can’t pinpoint if the differences have anything to do with drug exposure or have more to do with the tumors just being different to begin with.”
Alumkal’s sequential sampling method provided a much clearer picture of how enzalutamide resistance might emerge.
When they compared the baseline sample to the progression sample from the same patient, most tumors showed no significant gene expression changes. “That the gene expression program of a tumor prior to treatment looked very similar at progression while on enzalutamide is quite remarkable,” Alumkal says. “It speaks to how well most of the tumors were able to adapt and keep the androgen receptor engine on despite enzalutamide treatment.”
But that wasn’t the only surprise.
In three of the 21 cases, Alumkal and his team saw a profound shift in the wiring—or gene expression program—of the tumors.
“We knew that sometimes tumors become fuel-independent and no longer rely on the androgen receptor. These tumors instead turn on a gene expression program more common in nerve cells, rather than prostate cells, and shift to an aggressive form called neuroendocrine prostate cancer.”
However, Alumkal found that in 15 percent of cases, the tumors also became fuel-independent for another reason. “These tumors were wired in a unique way and were most consistent with a subtype of prostate cancer called double-negative prostate cancer, meaning the tumors no longer had the androgen receptor as an engine. But they also did not become neuroendocrine prostate cancer.”
Alumkal uses vehicles to describe this change.
“Initially, nearly all prostate tumors are gas guzzlers: very fuel dependent and powered by the androgen receptor as the engine. When treated with hormonal treatments, most tumors remain fuel-dependent but become more fuel efficient, able to go farther with less gasoline.
“Our work showed that the majority of the tumors—even after receiving enzalutamide—remain very fuel-dependent, which suggests that continuing to target the androgen receptor could make an enormous difference in these tumors,” Alumkal continued.
Alumkal found that three tumors converted to become double negative prostate cancer—akin to an electric vehicle. “The gasoline engine was replaced by a completely distinct set of machinery that allowed tumors to grow and survive,” Alumkal explained. The DNA mutations found in the baseline and progression biopsies from these converter tumors were the same, which strongly suggests that enzalutamide completely rewired the engine of the original fuel-dependent tumor to become fuel-independent at disease progression. “It’s a dramatic shift to wrap your head around.”
Although the baseline tumors appeared similar under the microscope, Alumkal’s team identified specific genes that were highly expressed in those that eventually became double-negative prostate cancer. This result suggests that certain tumors exist in a hybrid state, initially dependent on fuel but at risk for becoming a fuel-independent double negative prostate cancer during enzalutamide treatment.
Alumkal says results from the sequential sampling method suggest that enzalutamide is causing tumors to adapt, in some cases dramatically.
Alumkal notes that the gene signature he identified is preliminary, and the research team has more work to do. “Still, the fact that the DNA looks similar in the converters strongly indicates that enzalutamide is reprogramming tumors. We have more work to do, but it may be possible up-front to identify patients at greatest risk of having their tumor become fuel-independent after treatment with drugs like enzalutamide,” he said.
Reference: “Transcriptional profiling of matched patient biopsies clarifies molecular determinants of enzalutamide-induced lineage plasticity” 15 September 2022, Nature Communications.
DOI: 10.1038/s41467-022-32701-6
Funding: Stand Up To Cancer, Prostate Cancer Foundation, NIH/National Cancer Institute, NIH/National Cancer Institute, NIH/National Cancer Institute, NIH/National Cancer Institute, NIH/National Cancer Institute, NIH/National Cancer Institute, NIH/National Cancer Institute, NIH/National Cancer Institute, U.S. Department of Defense, U.S. Department of Defense, Doris Duke Charitable Foundation, The V Foundation, National Comprehensive Cancer Network, Astellas Pharma, The Sheppard Family Fund, University of Michigan Rogel Cancer Center
